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For the first
time Cryptosporidium can be automatically detected in less
than five minutes, after sample preparation, following the launch
of a new highly sensitive test from Chemunex. Such speed will
enable drinking water companies to streamline their quality control
procedures considerably, and afford greater protection to consumers
against this dangerous parasite. This is particularly pertinent
in view of the Drinking Water Inspectorate's (DWI) announcement
last week that inadequate treatment of drinking water for Cryptosporidium
could lead to criminal prosecution.
Amongst other
stringent requirements, the new DWI regulations require continuous
sampling and daily analysis of treated water for Cryptosporidium.
Current techniques, based on manual microscopy, typically take
30-120 minutes per sample for analysis, depending on the level
of debris present. In addition to being extremely time consuming,
the manual method is subject to error particular due to operator
fatigue associated with hours of microscope analysis.
By automating
the detection aspect of the testing using the ChemScan®RDI
analyser, one operator can run approximately 12-15 samples per
hour. The automated laser scanning of each sample is complete
in 3 minutes. With manual microscopy, this volume of samples could
take as much as six hours to analyse. Independent studies have
also shown the recovery of oocysts from spiked samples to be consistently
higher with the ChemScan system compared to manual microscopy.
The ChemScan
RDI provides an option to confirm the presence of Cryptosporidium
oocysts on the membrane filter using a microscope with a motorised
stage that is driven by the system. This automatically locates
the co-ordinates of suspected individual oocysts for visual validation,
typically taking 5 minutes. Once analysed, membranes can be stored
for re-analysis, if required.
This new rapid
technique represents the only automated Cryptosporidium
test available today. In addition to improving the sensitivity
of Cryptosporidium detection compared to manual microscopy,
it has the capability to significantly reduce manpower requirements
and costs, remove potential operator errors and allow increased
testing at times of need, such as following heavy rain fall and
outbreaks.
Cryptosporidium
has caused a number of contamination scares over recent years.
The parasite causes diarrhoea, nausea and vomiting, and is a major
public health concern since it can lead to death among individuals
with depressed immune systems, children or the elderly. One outbreak
in Milwaukee (USA) was responsible for 400,000 cases of infection
and caused 100 deaths. There have also been many smaller incidents
across Europe, notably on the outskirts of London in 1997, when
100,000 were affected.
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